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Curcumin is a chemical component extracted from the rhizome of some plants in the family coronaceae and araceae. Curcumin contains about 3% ~ 6%.
The ethanol solution of sample A should show pure yellow and light green fluorescence.If the ethanol extract is added with concentrated sulfuric acid, it will produce a deep purple color.Chromogenic reaction B an aqueous solution or dilute ethanol solution of the sample treated with hydrochloric acid until slightly orange at the beginning.The mixture is divided into two parts, one with a small amount of boric acid powder or crystal, and the part without boric acid turns significantly redder than the part without boric acid.Several pieces of filter paper can also be impregnated in the ethanol solution of pigment, dried at 100℃, and then moistened with a dilute solution of boric acid (with a few drops of hydrochloric acid).It should be cherry red when dry.
Chromatographic detection method:
5 ml of the test solution (0.01g sample dissolved in 1ml95% ethanol) was dripped on the TLC (microcrystalline cellulose, 0.1mm) and placed in the expansion tank containing 3-methyl-1-butanol/ethanol/water/ammonia (4:4:2:1) mixture, so that the solvent front increased by 10 ~ 15 cm.After a day and night observation under ultraviolet light: there are three yellow spots, Rf between 0.2 ~ 0.4;Rf of other spots is 0.6 ~ 0.8.All spots fluoresced yellow under ultraviolet light.6.95% ethanol has the maximum absorption peak at 425 nm.
Extraction process of curcumin:
The extraction methods of curcumin are various and have their own characteristics in the technological process, but generally speaking, curcumin can be extracted and refined.
The yield of curcumin from turmeric was increased by 8.1% compared with the traditional extraction method.The extraction process conditions were as follows: enzymatic hydrolysis temperature: 50℃; pH4.5; time: 120 min; enzyme concentration: 0.35 mg/ml.
HPLC determination method, curcumin extraction quantity as the index, orthogonal test method was used to optimize the extraction process, and it was concluded that the optimal extraction technology of curcumin by ultrasonic method was to add 8 times of alkaline water with pH value of 12, and extract for 4 times, each time 40 min.
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